Sequencing ServicesMetabolomics Testing and Profiling Services

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Metabolic profiling services offer phenotypic insights into the functional output of microbial communities, shedding light on metabolic processes and their impact on human health.

Metabolomics testing is the comprehensive study of metabolites, the small molecules intricately involved in the metabolic processes within biological systems. Through both Liquid Chromatography–Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectroscopy (GC-MS), CosmosID’s metabolite identification services are capable of detecting thousands of key metabolites and their respective pathways, including SCFA, Bile Acids, and even novel metabolite signatures.

In using metabolomics research methods, greater in-depth and multi-omic profiling of biological samples in any research condition can be achieved.

By delving into the metabolites produced and processed by the microbiome, we bridge the gap between microbes and their influence on human health. Contact us today to find out more about our metabolomics profiling services.

Metabolomics Services at CosmosID

The dedicated team at CosmosID extends a range of services to facilitate your metabolomics needs, accommodating both novices and experienced researchers. Collaborate directly with our specialized scientists who provide guidance on analytical chemistry, method selection, technical analysis details, and advanced data processing and interpretation.

Tailored to meet your specific needs, our state-of-the-art platform offers both untargeted and targeted metabolomics analyses, employing cutting-edge technologies such as gas chromatography (GC) and liquid chromatography (LC) coupled with mass spectrometry (MS) detectors.

Our instrument arsenal includes leading platforms like Thermo Scientific Q Exactive HF LC-MS/MS and Agilent Q GC-MS.

MetabolomicsFeatures of this service include:

Specialized sample processing
Liquid Chromatography–Mass Spectrometry (LC-MS) + Gas Chromatography-Mass Spectroscopy (GC-MS)
Untargeted Metabolomic Profiling + Relative Quantitation:
- Standard panels
  - SCFA
  - Bile Acid
  - Total Fatty Acid
  - Tryptophan
  - Amino Acids & TCA Cycle
  - HMO
- Custom panels
Comprehensive metabolomic reporting and interpretation services

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MetabolomicsUntargeted metabolomics

By utilizing multiple columns optimized for both polar and semi-polar compounds, our untargeted metabolomics services offer a global detection of diverse compound classes, providing a detailed snapshot of the metabolic status of microbial communities and host-microbe interactions.

Commonly detected compound classes include, but are not limited to, microbial metabolites, amino acids and derivatives, tryptophan metabolites, organic acids and short-chain fatty acids, lipids and lipid derivatives, nucleotides and nucleosides, and secondary metabolites.

This broad coverage ensures a comprehensive understanding of microbial capabilities, biomarkers of microbial activity, and the intricate interplay between the microbiome and host health.

Relevant compound classes commonly detected through untargeted metabolomics include:

Microbial Metabolites: Explore the metabolic landscape of microorganisms through the analysis of TMAO, bile acids, tryptophan, acylcarnitines, and more.
Amino Acids and Derivatives: Gain insights into microbial protein synthesis, nitrogen utilization, and nutrient availability.
Tryptophan Metabolites: Uncover the impact of indole, tryptamine, serotonin derivatives, and kynurenine pathway metabolites on host immune responses, gut-brain communication, inflammation, and neurodegeneration.
Organic Acids and Short-Chain Fatty Acids: Delve into the role of metabolites like acetate, propionate, and butyrate in gut health, immune modulation, and energy metabolism.
Lipids and Lipid Derivatives: Explore the contribution of lipids to microbial membrane composition, lipid metabolism, and interactions with host lipid metabolism.
Nucleotides and Nucleosides: Gain insights into microbial nucleotide metabolism and potential interactions with host nucleotide pools.
Secondary Metabolites: Identify antibiotics, toxins, and signaling molecules to understand microbial community dynamics and functional roles in the microbiome ecosystem.

MetabolomicsTargeted metabolomics

In addition to our untargeted metabolomics services, we offer targeted panels for dedicated analysis, allowing for a more focused exploration of specific metabolites. These include:

SCFA Panel

Acetic acid aka acetate (HMDB0000042)
Butanoic acid aka butyrate (HMDB0000039)
Heptanoic acid (HMDB0000666)
Hexanoic acid aka caproic acid (HMDB0000535)
Pentanoic acid aka valerate, valeric acid (HMDB0000892)
2-methylpropanoic acid aka isobutyrate, isobutyric acid (HMDB0001873)
3-Methylbutanoic acid aka isovalerate, isovaleric acid (HMDB0000718)
Formic acid aka formate (HMDB0000142)
Propanoic acid aka propionate (HMDB0000237)
4-Methylpentanoic acid aka isocaproic acid (HMDB0000689)

Bile Acid Panel

β-Muricholic acid (HMDB0000865)
Chenodeoxycholic acid (HMDB0000518)
Cholic acid aka cholate (HMDB0000619)
Deoxycholic acid aka deoxycholate (HMDB0000626)
Glycocholic acid (HMDB0000138)
Glycolithocholic acid (HMDB0000698)
Glycoursodeoxycholic acid (HMDB0000708)
Glycodeoxycholic acid (HMDB0000631)
Hyocholic acid (HMDB0000760)
Hyodeoxycholic acid (HMDB0000733)
Lithocholic acid aka lithocholate (HMDB0000761)
α-Muricholic acid (HMDB0000506)
Tauro-α-Muricholic acid (HMDB0258742)
Tauro-β-Muricholic acid (HMDB0000932)
Taurochenodeoxycholic acid (HMDB0000951)
Taurocholic acid (HMDB0000036)
Taurodeoxycholic acid aka taurodeoxycholate (HMDB0000896)
Taurohyodeoxycholic acid
Taurolithocholic acid (HMDB0000722)
Taurolithocholic acid sulfate (HMDB0002580)
Tauroursodeoxycholic acid (HMDB0000874)
Ursodeoxycholic acid (HMDB0000946)
ω-Muricholic acid (HMDB0000364)
Isolithocholic acid (HMDB0000717)
12-Ketolithocholic acid (HMDB0000328)

Total Fatty Acid Panel

⍺-Linolenic acid (HMDB0001388)
11,14-Eicosadienoic acid (HMDB0244372)
13,16-Docosadienoic acid (HMDB0244521)
Arachidic acid (HMDB0002212)
Arachidonic acid (HMDB0001043)
cis-10-Heptadecenoic acid (HMDB0060038)
cis-10-Pentadecenoic acid (HMDB0304816)
cis-11-Eicosenoic acid (HMDB0002231)
Decanoic acid aka caprate, capric acid (HMDB0000511)
Dihomo-⍺-linolenic acid (HMDB0060039)
Dihomo-γ-linoleic acid (HMDB0002925)
Docosahexaenoic acid (HMDB0002183)
Docosanoic acid (HMDB0000944)
Docosapentaenoic acid (HMDB0006528)
Dodecanoic acid (HMDB0000638)
Eicosapentaenoic acid (HMDB0001999)
Elaidic acid (HMDB0000573)
Erucic acid (HMDB0002068)
Heneicosanoic acid (HMDB0002345)
Heptadecanoic acid (HMDB0002259)
Linoleic acid (HMDB0000673)
Linolelaidic acid (HMDB0006270)
Myristic acid (HMDB0000806)
Myristoleic acid (HMDB0002000)
Nervonic acid (HMDB0002368)
Octadecanoic acid (HMDB0000827)
Oleic acid (HMDB0000207)
Palmitic acid (HMDB0000220)
Palmitoleic acid (HMDB0003229)
Pentadecanoic acid (HMDB0000826)
Tetracosanoic acid (HMDB0002003)
Tricosanoic acid (HMDB0001160)
Tridecanoic acid (HMDB0000910)
Undecanoic acid (HMDB0000947)
γ-Linolenic acid (HMDB0003073)

Tryptophan Panel

2,3-Pyridinedicarboxylic acid (HMDB0000232)
2-Picolinic acid (HMDB0002243)
3-(4-hydroxyphenyl)propionic acid (HMDB0002199)
3-Methylindole (HMDB0000466)
Anthranilic acid (HMDB0001123)
Indole (HMDB0000738)
Indole-3-acetic acid (HMDB0000197)*
Indole-3-acrylic acid (HMDB0000734)
Indole-3-butyric acid (HMDB0002096)
Indole-3-propionic acid (HMDB0002302)
Indole-3-pyruvic acid (HMDB0060484)*
Tryptamine (HMDB0000303)
2-Oxoglutaric acid (HMDB0000208)
5-Aminovaleric acid (HMDB0003355)
Alanine (HMDB0000161)
Asparagine (HMDB0000168)
Aspartic acid (HMDB0000191)
cis-Aconitic acid (HMDB0000072)
Citric acid (HMDB0000094)
Cystine (HMDB0000192)
Fumaric acid aka fumarate (HMDB0000134)
Glutamic acid aka glutamate (HMDB0000148)
Glutamine (HMDB0000641)
Glycine (HMDB0000123)
Histidine (HMDB0000177)
Isocitric acid (HMDB0000193)
Isoleucine (HMDB0000172)
Lactic acid aka lactate (HMDB0000190)
Leucine (HMDB0000687)
Lysine (HMDB0000182)
Malic acid (HMDB0000156)
Methionine (HMDB0000696)
Ornithine (HMDB0000214)
Phenylalanine (HMDB0000159)
Phosphoenolpyruvic acid (HMDB0000263)
Proline (HMDB0000162)
Serine (HMDB0000187)
Succinic acid aka succinate (HMDB0000254)
Threonine (HMDB0000167)
Tryptophan (HMDB0000929)
Tyrosine (HMDB0000158)
Valine (HMDB0000883)

*NOTE: Indole-3-acetic acid and indole-3-pyruvic acid co-elute; their peaks can therefore not be resolved and they cannot be quantified individually. These compounds are marked with a ‘*’ in compounds detected/quantified section.

Amino Acids & TCA Cycle Intermediates Panel

2-Oxoglutaric acid (HMDB0000208)
5-Aminovaleric acid (HMDB0003355)
Alanine (HMDB0000161)
Asparagine (HMDB0000168)
Aspartic acid (HMDB0000191)
cis-Aconitic acid (HMDB0000072)
Citric acid (HMDB0000094)
Cystine (HMDB0000192)
Fumaric acid aka fumarate (HMDB0000134)
Glutamic acid aka glutamate (HMDB0000148)
Glutamine (HMDB0000641)
Glycine (HMDB0000123)
Histidine (HMDB0000177)
Isocitric acid (HMDB0000193)
Isoleucine (HMDB0000172)
Lactic acid aka lactate (HMDB0000190)
Leucine (HMDB0000687)
Lysine (HMDB0000182)
Malic acid (HMDB0000156)
Methionine (HMDB0000696)
Ornithine (HMDB0000214)
Phenylalanine (HMDB0000159)
Phosphoenolpyruvic acid (HMDB0000263)
Proline (HMDB0000162)
Serine (HMDB0000187)
Succinic acid aka succinate (HMDB0000254)
Threonine (HMDB0000167)
Tryptophan (HMDB0000929)
Tyrosine (HMDB0000158)
Valine (HMDB0000883)

Human Milk Oligosaccharides (HMOs)

Most HMOs or Human Milk Oligosaccharides are measured as prebiotics in formula or as compounds in breastmilk. Contact our experts today to find the HMOs that best fit your study goals.

MetabolomicsCombine the Power of Metabolomics and Metagenomics with CosmosID

Metabolomics data is a crucial complement to microbiome research, providing phenotypic information for investigating mechanisms in microbiome studies.

Contact us today to discuss how our metabolomics analysis can enhance your understanding of the microbiome and integrate seamlessly with metagenomics data.

Explore our multi-omics solutions that seamlessly integrate metabolomics data with metagenomics data, unlocking a deeper understanding of the complex interactions within biological systems.

Embark on a transformative scientific journey with CosmosID, where metabolomics meets precision and innovation.

Metabolomics Services Frequently Asked Questions

What are the uses of metabolomics?

Metabolomics has various uses, including biomarker discovery, investigating drug metabolism, understanding biological processes, and studying diseases such as diabetes, cardiovascular diseases, and neurological disorders. Additionally, metabolomics can be leveraged with additional “-omics”, such as metagenomics, to provide another data layer into understanding the connection between the genotype and phenotype.

What are the types of metabolomics?

There are two types of metabolomics: targeted and untargeted. In targeted metabolomics, specific metabolites are measured in order to answer specific questions. In untargeted metabolomics, a broad range of metabolites is measured in order to gain an understanding of the complex biochemical interactions taking place in a sample.

What is the difference between untargeted and targeted metabolomics?

Untargeted metabolomics is a process in which retention time, mass, and fragmentation pattern of and/all features that are detected during testing. This allows the data to detect a wide range of metabolites for characterization, including the possibility of novel compound discovery.

Targeted metabolomics involves the utilization of a predetermined panel of compounds in which testing will be performed on. In using these predetermined compounds during testing, calibration curves are generated which enables the detection and absolute quantification of all metabolites within the panel. While targeted metabolomics does not provide information on metabolite presence outside the panel, it provides a more sensitive and absolute measurement of the panel compounds within each sample.

How much does metabolomics service cost?

The cost of metabolomics can vary depending on the complexity of the analysis and the number of samples, which ranges from $100 to $500. However, it is important to note that metabolomics has become more affordable in recent years due to advances in instrumentation and automation.

What are some examples of metabolomics?

Examples of metabolomics studies include identifying metabolic changes in cancer cells, studying the effects of drugs on metabolic pathways, and investigating the metabolic profiles of tissue samples from a biological system.

Other examples of metabolomics include studying physiological processes, such as the response of the body to exercise and nutrition. Furthermore, metabolomics can detect biomarkers that can be used in early diagnosis and treatment monitoring.