Sequencing ServicesMetabolomics Testing and Services


In addition to genomics, CosmosID offers metabolomic services optimized for microbiome research, thus enabling the interrogation of the underlying phenotypic profile in relation to the microbiome. 

Through both Liquid Chromatography–Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectroscopy (GC-MS), CosmosID’s metabolomic services are capable of detecting thousands of metabolites, including SCFA, Bile Acids, and even novel metabolite signatures. In using this approach, greater in-depth and multi-omic profiling of the microbiome can be achieved.

MetabolomicsFeatures of this service include:

  • Specialized sample processing
  • Liquid Chromatography–Mass Spectrometry (LC-MS) + Gas Chromatography-Mass Spectroscopy (GC-MS)
  • Untargeted Metabolomic Profiling + Relative Quantitation
  • Targeted Metabolomics Profiling + Relative AND Absolute Quantitation (SCFA & Select Metabolites)
  • Comprehensive metabolomic reporting and interpretation services

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MetabolomicsSample types currently offered for metabolomics testing at CosmosID include:

  • Stool
  • Urine
  • Blood/Plasma
  • Skin
  • Bacterial Isolates

MetabolomicsMetabolite identification classes detected by the CosmosID metabolomics method include:

  • Acetylated amino acids
  • Amines and amides
  • Bile acids
  • Carbohydrate phosphate
  • Catecholamines
  • Coumarins
  • D-Amino acids
  • Dicarboxylic fatty acids
  • Dipeptides
  • Disaccharides
  • Environment
  • Fatty acid esters (other)
  • Fatty acid methyl esters
  • Fatty alcohol derivatives
  • Fatty alcohols (normal)
  • Fatty ketones (normal)
  • FDA Approved drugs
  • Flavonoids
  • Glycerolipids
  • Glycerophospholipids
  • Indoles
  • L-Amino acids
  • Mono-carboxilic fatty acids
  • Monosaccharides
  • Naphthalenes
  • Nucleotides
  • Nucleosides
  • Other poly-ols
  • Phenols and benzyls
  • Polyphenols
  • Preservatives and antioxidants
  • Purines and Pyrimidines
  • Short-chain-carboxylic acids
  • Short-chain fatty acids
  • Short-chain mono-carboxilic acids
  • Steroids
  • Sweeteners
  • Synthetic additives
  • Terpenes and terpenoids
  • Tri-carboxylic acids
  • Vitamins_CoFactors
  • Xanthines

MetabolomicsWhy Choose Us?

Our highly experienced and knowledgeable experts customize your metabolomics testing and data analysis needs. Furthermore, our team of scientists are able to provide expert guidance in interpretation and reporting for more meaningful insights for your research.

It is our priority to provide world-class metabolomics services, offering reliable quantitative analysis, results and insights with the highest quality standards. Additionally – you get all the data. With metabolomics at CosmosID, we offer a transparent process that allows you to have maximum confidence in results, which includes providing you with the raw MS/MS data from your samples.

From sample preparation through to data interpretation, our approach can support you with biomarker discovery, product development, and clinical research. With our metabolomics services, you can maximize the understanding of your data for efficient decision-making.

Our team will work with you to develop a comprehensive analysis plan that meets your individual needs and goals, offering competitive prices for our services and guaranteed turn-around times of results.

We understand the importance of having accurate, quantitative data for decision making. Contact us today to get started on your metabolomics testing journey!

MetabolomicsUntargeted vs Targeted - Which is right for you?

At CosmosID, a combination of LC-MS and GC-MS is deployed for sample testing. 

In using this technique, CosmosID is able to provide metabolomics profiling in an “untargeted” approach, enabling the capture and detection of as many possible metabolites within each sample. 

By combining retention time, mass, and MS fragmentation, metabolites are profiled one by one as they are eluted through their respective MS test and compiled into a comprehensive report of thousands of metabolites.. 

Within this “untargeted” test report, a list of all detected metabolites as well as their relative concentrations within the sample is provided. This is a great technique for an explorative look into the total metabolite profile of each sample, however the comparison of metabolite levels from sample to sample is strictly on a relative basis.

With “targeted” testing, a list of known metabolites of interest must be established prior to testing. CosmosID has the capability to tailor testing to client specific needs, including absolute quantification, of SCFA as well as limited sample-specific metabolites.. 

By implementing absolute quantification, a known and comparable unit of measurement (such as micromol/g) can be recorded, thus enabling your metabolites of interest to be compared from sample to sample on an absolute basis.

MetabolomicsSample Processing

CosmosID’s CLIA-certified, GCP compliant facility* receives samples from all around the world. Your precious pre-clinical or clinical samples will be barcoded & accessioned into the CosmosID LIMS system for sample tracking and project management.

CosmosID will perform specialized sample processing optimized by sample type, resulting in minimal bias, optimum yield and purity.

MetabolomicsLiquid Chromatography–Mass Spectrometry (LC-MS) + Gas Chromatography-Mass Spectroscopy (GC-MS) Processing:

At CosmosID, both Liquid Chromatography–Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectroscopy (GC-MS) are used for greater metabolite coverage in your samples.

With LC-MS, we use the sample in liquid state and push it through a solid column with compounds on the membrane. As the sample is processed, metabolites are eluted one by one, at which point their retention time, mass, and MS fragmentation is recorded for identification and quantification.

With GC-MS, instead of pushing liquid through the column, the sample is put in a gas state and pushed through and interacts with a liquid film. By interacting with the liquid film, metabolites are separated and eluted one by one, at which point their retention time, mass, and MS fragmentation is recorded for identification and quantification.

MetabolomicsMetabolomic Analysis:

At CosmosID, metabolomic signatures are analyzed and detected through a variety of databases for the most comprehensive metabolite profile of your samples. These databases include our internal reference library, GNPS, NIST2020, and proprietary In Silico Matching techniques (including a query of the PubChem metabolite library).

MetabolomicsTurnaround Time and Customer Service:

CosmosID offers different turnaround times between 2-8 weeks, depending on the total sample number and other client needs. Simply let us know about any special requirements and we can tailor our service accordingly.

Once samples have arrived at the lab, our operations team will keep you updated on a weekly basis on the status of your study!

Metabolomics Services Frequently Asked Questions

What are the uses of metabolomics?

Metabolomics has various uses, including biomarker discovery, investigating drug metabolism, understanding biological processes, and studying diseases such as diabetes, cardiovascular diseases, and neurological disorders. Additionally, metabolomics can be leveraged with additional “-omics”, such as metagenomics, to provide another data layer into understanding the connection between the genotype and phenotype. 

What are the types of metabolomics?

There are two types of metabolomics: targeted and untargeted. In targeted metabolomics, specific metabolites are measured in order to answer specific questions. In untargeted metabolomics, a broad range of metabolites is measured in order to gain an understanding of the complex biochemical interactions taking place in a sample.

What is the difference between untargeted and targeted metabolomics?

Untargeted metabolomics is a process in which retention time, mass, and fragmentation pattern of and/all features that are detected during testing. This allows the data to detect a wide range of metabolites for characterization, including the possibility of novel compound discovery. 

Targeted metabolomics involves the utilization of a predetermined panel of compounds in which testing will be performed on. In using these predetermined compounds during testing, calibration curves are generated which enables the detection and absolute quantification of all metabolites within the panel. While targeted metabolomics does not provide information on metabolite presence outside the panel, it provides a more sensitive and absolute measurement of the panel compounds within each sample.

How much does metabolomics service cost?

The cost of metabolomics can vary depending on the complexity of the analysis and the number of samples, which ranges from $100 to $500. However, it is important to note that metabolomics has become more affordable in recent years due to advances in instrumentation and automation.

What are some examples of metabolomics?

Examples of metabolomics studies include identifying metabolic changes in cancer cells, studying the effects of drugs on metabolic pathways, and investigating the metabolic profiles of tissue samples from a biological system.

Other examples of metabolomics include studying physiological processes, such as the response of the body to exercise and nutrition. Furthermore, metabolomics can detect biomarkers that can be used in early diagnosis and treatment monitoring.