In shotgun metagenomics, whole DNA is extracted from a sample then exposed to random fragmentation prior to NGS. By taking an untargeted approach to sequencing whole DNA and avoiding primer-dependent, PCR Amplification like in amplicon sequencing services, one gains an exponential amount of data and advantages alongside it.
Given the ability to provide not only very detailed information on taxonomy, but also the functional potential of a metagenome, this method is unparalleled for correlating with multi-omics, metadata and other study variables to unlock the microbiome.
Common applications include, but are not limited to:
CosmosID’s CLIA-certified, GCP compliant facility receives samples from all around the world. Whether you’re batch sending samples for a microbiome R&D project or coordinating with multiple clinical trial sites, your precious samples will be barcoded & accessioned into the CosmosID LIMS system for sample tracking and project management purposes.
CosmosID also offers validated DNA extraction protocols optimized for shotgun sequencing and based on sample type, resulting in minimal bias, optimum yield and purity for your most prioritized organisms.
CosmosID’s CLIA-certified, GCP compliant facility receives samples from all around the world. Whether you’re batch sending samples for a microbiome R&D project or coordinating with multiple clinical trial sites, your precious samples will be barcoded & accessioned into the CosmosID LIMS system for sample tracking and project management purposes.
CosmosID also offers validated DNA extraction protocols optimized for shotgun sequencing and based on sample type, resulting in minimal bias, optimum yield and purity for your most prioritized organisms.
Once DNA QC metrics are established for received or generated DNA samples, these samples then undergo library preparation & QC prior to sequencing on a range of different instruments, depending on study question & client preference:
In addition to this, CosmosID also offers a range of different sequencing depths from 6M to 100M reads with different run-type/flowcell options.
In addition to sample processing & sequencing, CosmosID includes, as standard, access to the CosmosID -Hub where you can expect:
Industry-leading species & strain-level resolution of bacteria, viruses, bacteriophages, fungi & protists
Access to AMR & Virulence Analysis
Interactive and user-friendly charts & visualizations with access to comparative analysis features including heat maps, PCA plots, Alpha Diversity & Beta Diversity PCoA
Methods & QC data upon request – DNA QC, Library QC & fastQC
Fastq/Fasta file transfer via AWS s3 Bucket/Dropbox
Deadline coming up? CosmosID offers different turnaround time options as fast as 3-5 business days for shotgun sequencing & genome analysis. Simply let us know about any special requirements and we can tailor our complex genome shotgun sequencing services accordingly.
Once samples have arrived at the lab, our operations team will keep you updated on a weekly basis on the status of your study and the rapid and sensitive classification of your metagenomic sequences. Request a quote for expert human genome sequence research and analysis from the microbial community today!
We will consult on sequencing depth based upon three main criteria:
A wide range of depths are readily available at CosmosID, from 3M to 100M total reads and beyond, if necessary, with exact specifications available upon request.