Sequencing ServicesBacterial Isolate Sequencing
CosmosID offers end-to-end whole genome sequencing (WGS) and analysis of microbial isolates. DNA Extraction, Library preparation and Next-Generation Sequencing (NGS) is carried out in our CLIA-certified facilities. For data interpretation we use an in-house bioinformatics platform that is built around CosmosID’s class-leading databases and has been optimized for isolate whole genome data.
Bacterial Isolate SequencingIs Whole-Genome Sequencing right for you?
WGS of bacterial isolates offers unique advantages over traditional typing methods and is now available at very affordable rates. Unlike PCR, this affordable technology reveals the complete genetic makeup of an organism and offers unprecedented insight. A single assay reveals:
- Unambiguous taxonomic classification
- Highly accurate depiction of strain relatedness, pathogenesis and transmission
- Functional characteristics like antimicrobial resistance, virulence, and metabolic capabilities
Bacterial Isolate SequencingApplications
There are numerous applications of WGS of isolates, given the ability to reveal detailed taxonomic, phylogentic and functional output. CosmosID has extensive experience in:
- Live Biotherapeutic & Probiotic Characterization
- Hospital-Acquired Infections (HAI’s) & Clinical Microbiology
- Strain Sub-Typing & Epidemiology
- Food Safety Testing
- Agricultural & Environmental Research
- Academic Research
Bacterial Isolate SequencingWhat type of analysis is included?
- Raw data via AWS Transfer
- Data QC, Genome Assembly, Strain ID, SNP Analysis & Phylogenetic Trees
- CosmosID Analysis Report
- Data consultation & walk-through
- Characterization – AMR, Virulence, MLST, Average Nucleotide Identity & Functional Annotation
- Custom Bioinformatics available on request
How do I get started?
Customize your isolate sequencing:
- Choose your sequencing technology – Short & Long-Reads available.
- Choose your turnaround time – As fast as 3 days.
- Highlight your bioinformatics & reporting requirements.
Then mail us your isolate cell pellets or nucleic acid!
Sample Processing at CosmosID’s CLIA certified and GCP Laboratory.
Frequently Asked Questions
What common applications are there for isolate sequencing & analysis?
We support a range of academic and industrial applications including, but not limited to, probiotic/LBP strain characterization, epidemiology, infectious disease research, food safety testing, manufacturing QC and many others!
How much does isolate WGS cost?
Prices vary depending on the specifics of your study and analytical requirements, starting from just $99 USD per isolate, including primary bioinformatic analysis
How long will the process take?
We offer a range of different turnaround time options to suit your project needs with sequencing and analysis being as fast as 3-5 business days.
Do you have the ability to sequence and analyze any species?
Bacterial and fungal organisms differ tremendously based on their cell wall thickness, genome size & GC content. As such, some organisms require a slightly more specialized approach when it comes to DNA extraction, library preparation techniques, targeted coverage and analysis strategy. Once we receive a list of your organisms of interest, we’ll assess these nuances and get back to you with a proposed plan of action. Nonetheless, by assembling genomes de novo, this is well suited to novel strain discovery in a wide range of applications.
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