Each participating laboratory receives its DNA mixture from NIST free of charge. Participants perform a library prep and sequencing using their preferred library prep method and sequencing platform.


For this challenge, in order to make data cross-comparable, sequencing techniques must be SHOTGUN METAGENOMIC approaches (NOT 16s amplicon).


The DNA Mixture: To be Sequenced by Participants

Participants use their preferred choice of library prep and NGS technology

Output to submit: FASTQ Raw Data and Metadata:
Library Prep and Sequencing Parameters