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When using metagenomic methods to assess the content of a complex microbial sample, there are many steps in the measurement process where bias might be introduced; including, but not limited to: sample collection and storage, DNA extraction method, library construction and/or PCR, NGS platform, raw data filtering and data analysis/interpretation. 


Investigating and understanding the source and extent of measurement bias is often accomplished through the development and utilization of a shared reference material (“ground truth”) followed by repeated measurements of the material in multiple independent laboratories. 


Welcome to The NIST-ABRF-CosmosID Metagenomic MVP Challenge v1.0


We have a metagenomic reference material (“ground truth”) and you are the independent laboratories! This challenge is open to the community at-large, but we are limiting the number of participants to the first 80 who sign-up. 


This Challenge (v1.0) is intended to be the first of many challenges that will each address a different step of the measurement process.  The goal of Challenge v1.0 is to specifically identify and understand measurement bias associated with 1) shotgun library preparation techniques and 2) next-generation sequencing platforms.   We recognize that this only addresses a small aspect of the larger measurement process.  This is intentional and it is our intent to address other steps of the measurement process in future challenges.